Open circuit potential (OCP)Open circuit conditions impose the highest external resistance on the cells, resulting in a uniform biofilm morphology and ample time for substrate diffusion into the biofilm33. As applied external resistance progressed the oxidation reaction of the organic substrate by biocatalysts to produce significantly more electrons, the biodegradation reaction proceeded with the least amount of driving force possible under open circuit conditions. As a result, the bacteria have sufficient time to form a uniform biofilm. The formed biofilm will be homogeneous, which allows for easier access to the substrate than the heterogeneous biofilm formed under closed circuit conditions. The initial assessment of Zn, Al, Sn, Cu, and Ni was accomplished by tracking the OCP evolution of E. coli and S. oneidensis MR-1 under the same injection condition (0.2 ml h-1 flow rate) and…
